Anti-ATP5O [4C11C10D12] antibody (ab110276)抗ATP5O [4C11C10D12]抗體產(chǎn)品信息：

• Product nameAnti-ATP5O [4C11C10D12] antibodySee all ATP5O primary antibodies ...
• Description

  Mouse monoclonal [4C11C10D12] to ATP5O

• Tested applicationsICC/IF, WB, Flow Cyt, IHC-P more details
• Species reactivity

  Reacts with: Cow, Human, African Green Monkey

• Immunogen

  Recombinant mature Human ATP5O.

• Positive control
  • Isolated mitochondria from Human heart and Bovine heart, Cultured Human embryonic lung-derived fibroblasts (strain MRC5), Human colon tissue, HL-60 cells

Properties

• FormLiquid
• Storage instructionsStore at +4°C. Do not freeze.
• Storage bufferPreservative: 0.02% Sodium azide
  Constituent: HBS

• Concentration 100 µg at 1 mg/ml

• PurityIgG fraction
• Purification notesNear homogeneity as judged by SDS-PAGE. ab110276 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
• Clonality Monoclonal
• Clone number4C11C10D12
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Tags & Cell Markers
  • Subcellular Markers
  • Organelles
  • Mitochondria
  • Signal Transduction
  • Metabolism
  • Mitochondrial
  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers
  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Oxidative phosphorylation
  • Complex V
  • Metabolism
  • Types of disease
  • Cancer

Applications

Our Abpromise guarantee covers the use of ab110276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

• FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
• Sequence similaritiesBelongs to the ATPase delta chain family.
• Cellular localizationMitochondrion. Mitochondrion inner membrane.
• Information by UniProt

• Database links

  • Entrez Gene: ATP5O Cow
  • Entrez Gene: 539 Human
  • Omim: 600828 Human
  • SwissProt: P13621 Cow
  • SwissProt: P48047 Human
  • Unigene: 49172 Cow
  • Unigene: 409140 Human

• Alternative names

  • ATP synthase O subunit mitochondrial precursor antibody
  • ATP synthase subunit O antibody
  • ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit antibody

Anti-ATP5O [4C11C10D12] antibody images

• 

  Western blot - ATP5O antibody [4C11C10D12] (ab110276)

  All lanes : Anti-ATP5O [4C11C10D12] antibody (ab110276) at 0.5 µg/ml
  Lane 1 : Human heart mitochondria at 5 µg
  Lane 2 : Bovine heart mitochondria at 1 µg
  Predicted band size : 23 kDa

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ATP5O antibody [4C11C10D12] (ab110276)

  Human colon tissue fixed with 4% PFA and antibody detection with ab110276 using the ABC system. ab110276 diluted 1/500 and incubated for 1 hour. Sections were incubated in peroxidase-conjugated rabbit anti-mouse immunoglobulins (diluted 1/100 in 4% BSA in PBST) for 1 hour at room temp.

• 

  Immunocytochemistry/ Immunofluorescence - ATP5O antibody [4C11C10D12] (ab110276)

  Mitochondrial localization of ATP5O. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110276 (0.5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml).

• 

  Flow Cytometry - ATP5O antibody [4C11C10D12] (ab110276)

  HL-60 cells were stained with 1 µg/mL ab110276 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

• 

  Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276)

  All lanes : Anti-ATP5O [4C11C10D12] antibody (ab110276) at 1 µg/ml
  Lane 1 : HDFn (human) cell lysates at 20 µg
  Lane 2 : COS7 (monkey) cell lysates at 20 µg
  Lane 3 : H4IIE (rat) cell lysates at 20 µg
  Lane 4 : MEF (mouse) cell lysates at 20 µg
  Lane 5 : bovine heart mitochondria lysates at 5 µg
  Predicted band size : 23 kDa

  Secondary antibody: Goat-Anti-Mouse-IR800 1:4000

公司介紹：

    Abcam位于英國(guó)的劍橋科學(xué)園，成立于1998年，專(zhuān)門(mén)生產(chǎn)和分銷(xiāo)研究型抗體。主要?jiǎng)?chuàng)立人Jonathan Milner和實(shí)驗(yàn)室同事構(gòu)思成立一間了解科研人員需要的網(wǎng)上抗體公司，理念是Abcam銷(xiāo)售質(zhì)量高的抗體，并提供zui全面、誠(chéng)實(shí)、時(shí)新的說(shuō)明書(shū)，快速的送貨服務(wù)，及高效率的顧客和服務(wù)。我們的在線目錄(www.obgz.cn) 已有差不多100,000種抗體和試劑，并不斷添加，供應(yīng)予百多個(gè)國(guó)家。Abcam于2005年11月在倫敦證券交易所上市，在美國(guó)馬薩諸塞州、日本東京及香港均設(shè)有分公司。

    Abcam 一直致力加強(qiáng)產(chǎn)品線，為使研究員更容易找到蛋白質(zhì)研究試劑產(chǎn)品，在2011并購(gòu)了美國(guó)的MitoSciences公司，加強(qiáng)了免疫分析方面的產(chǎn)品供應(yīng)；同年也并購(gòu)了英國(guó)的Ascent Scientific 公司，開(kāi)展了生化試劑的供應(yīng)。在2012年，Abcam并購(gòu)了美國(guó)的Epitomics 公司，成為一家有的RabMAbs® 供應(yīng)商。

    Abcam 的 目標(biāo)是給世界上的抗體建立zui大的在線目錄，為各地科學(xué)家提供*產(chǎn)品，成為各國(guó)科學(xué)界的重要伙伴。我們?yōu)樗挟a(chǎn)品提供來(lái)使客戶獲得預(yù)期的結(jié) 果。為提供高質(zhì)量的抗體來(lái)指向盡可能多的靶蛋白，我們盡所能在盡可能多的應(yīng)用和物種中檢測(cè)每種抗體。我們相信誠(chéng)信才是上策，在我們的上會(huì)發(fā)布有關(guān)我們 每一種產(chǎn)品的盡可能多的信息。

更多產(chǎn)品：

Abcamab1132Mouse monoclonal [1718] to Thyroid Hormone Receptor100ul3996Thyroid Hormone Receptor抗體一抗

Abcamab11320Rabbit polyclonal to gamma Tubulin - Centrosome Marker100ul3996gamma Tubulin抗體一抗

Abcamab11321Rabbit polyclonal to gamma Tubulin - Centrosome Marker100ul3996gamma Tubulin抗體一抗

Abcamab11322Mouse monoclonal [TUB-11] to epsilon Tubulin100ul3996epsilon Tubulin抗體一抗

Abcamab11323Mouse monoclonal [6-11B-1] to Tubulin （acetyl K40）100ul3996Tubulin抗體一抗

Abcamab11324Mouse monoclonal [B3] to Tubulin - Polyglutamylated100ul4274Tubulin抗體一抗

Abcamab11325Mouse monoclonal [TUB-1A2] to Tubulin100ul3996Tubulin抗體一抗

Abcamab11326Mouse monoclonal [100/2] to alpha Adaptin100ul3996alpha Adaptin抗體一抗

Abcam  ab11414Mouse monoclonal [SN6] to CD105100ug3996CD105hystemcell一抗

Abcam  ab11415Mouse monoclonal [SN6] to CD105 （FITC）100ug5029CD105hystemcell一抗

Abcam  ab11417Mouse monoclonal [D9D10] to Interferon gamma （FITC）50ug3996IFN-gammahystemcell一抗

Abcam  ab11418Mouse monoclonal [Z-ZE4] to IGFBP150ug3996IGFBP1hystemcell一抗

Abcam  ab11419Mouse monoclonal [9C8] to GADD15350ug4418GADD153hystemcell一抗

Abcam  ab1142Rabbit polyclonal to Bcl10100ul3996Bcl-1hystemcell一抗

Abcam  ab11420Rabbit polyclonal to ERp2950ul3996ERp29hystemcell一抗

Abcam  ab11421Rabbit polyclonal to ERp5750ul3996ERp57hystemcell一抗

Abcam  ab11422Rabbit polyclonal to ERp7250ug3996ERp72hystemcell一抗

Abcam  ab11423Mouse monoclonal [1234] to Rapsyn50ug3996Rapsynhystemcell一抗

Abcam  ab11424Rabbit polyclonal to EDG150ug3996EDG1hystemcell一抗

Abcam  ab11425Mouse monoclonal [1351] to Syntrophin50ug3996Syntrophinhystemcell一抗

Abcam  ab11426Rabbit polyclonal to Calbindin - Neuronal Marker50ug3996Calbindinhystemcell一抗

Abcam  ab11427Rabbit polyclonal to Parvalbumin50ug3996Parvalbuminhystemcell一抗

Abcam  ab11428Rabbit polyclonal to S100 alpha50ug3996S100 alphahystemcell一抗

Abcam  ab11429Rabbit polyclonal to RELM beta50ul3996RELM betahystemcell一抗

Abcam  ab1143Rabbit polyclonal to Acinus100ul3996Acinushystemcell一抗

Abcam  ab11430Rabbit polyclonal to Resistin50ul3996Resistinhystemcell一抗

Abcam  ab11431Rabbit polyclonal to Resistin50ug3996Resistinhystemcell一抗

Abcam  ab11432Rabbit polyclonal to PDIA650ul3996P5hystemcell一抗

Abcam  ab11433Mouse monoclonal [5] to VCP50ul4418VCPhystemcell一抗

Abcam  ab11434Rabbit polyclonal to Ataxin 750ug3996Ataxin 7hystemcell一抗

Abcam  ab11436Rabbit polyclonal to PSMD750ug3996Proteasome 19S S12hystemcell一抗

Abcam  ab11437Rabbit polyclonal to Proteasome 20S alpha 550ug3996Proteasome 20S alpha 5hystemcell一抗

Abcam  ab11438Mouse monoclonal [LZA2] to Listeria monocytogenes250ug3996Listeria monocytogeneshystemcell一抗

Abcam  ab11439Mouse monoclonal [LZH1] to Listeria monocytogenes250ug3996Listeria monocytogeneshystemcell一抗

Abcam  ab1144Rabbit polyclonal to Acinus100ul3996Acinushystemcell一抗

Abcam  ab11441Mouse monoclonal [LX32] to Listeria monocytogenes250ug3996Listeria monocytogeneshystemcell一抗

Abcam  ab11442Mouse monoclonal [HBe7] to Hepatitis B Virus E Antigen250ug3996HBeAghystemcell一抗

Abcam  ab11443Mouse monoclonal [HBe3] to Hepatitis B Virus E Antigen （HRP）200ug3996HBeAghystemcell一抗

Abcam  ab11444Mouse monoclonal [HBe8 ] to Hepatitis B Virus E Antigen500ug3996HBeAghystemcell一抗

Abcam  ab11447Mouse monoclonal [B-G5 ] to IL2 （FITC）50ug3996IL-2hystemcell一抗

Abcam  ab11448Rat monoclonal [MQ1-17H12] to IL2250ug3996IL-2hystemcell一抗

Abcam  ab11449Mouse monoclonal [B-E8 ] to IL6100ug3996IL-6hystemcell一抗

Abcam  ab1145Rabbit polyclonal to FLASH100ul3996FLASHhystemcell一抗

Abcam  ab11450Mouse monoclonal [B-K8 ] to IL8100ug3996IL-8hystemcell一抗

Abcam  ab11451Mouse monoclonal [4D5] to IP10250ug3996IP-10hystemcell一抗

Abcam  ab11453Mouse monoclonal [h.mcp.3] to MCP3250ug3996MCP-3hystemcell一抗

Abcam  ab11456Mouse monoclonal [1E8-269] to TGF alpha1ml3996TGF alphahystemcell一抗

Abcam  ab11457Mouse monoclonal [B-D9] to TNF alpha （FITC）50ug3996TNF-alphahystemcell一抗

Abcam  ab1132Mouse monoclonal [1718] to Thyroid Hormone Receptor100ul3996Thyroid Hormone Receptorhystemcell一抗

Abcam  ab11320Rabbit polyclonal to gamma Tubulin - Centrosome Marker100ul3996gamma Tubulinhystemcell一抗

Abcam  ab11321Rabbit polyclonal to gamma Tubulin - Centrosome Marker100ul3996gamma Tubulinhystemcell一抗

Abcam  ab11322Mouse monoclonal [TUB-11] to epsilon Tubulin100ul3996epsilon Tubulinhystemcell一抗

Abcam  ab11323Mouse monoclonal [6-11B-1] to Tubulin （acetyl K40）100ul3996Tubulinhystemcell一抗

Abcam  ab11324Mouse monoclonal [B3] to Tubulin - Polyglutamylated100ul4274Tubulinhystemcell一抗

Abcam  ab11325Mouse monoclonal [TUB-1A2] to Tubulin100ul3996Tubulinhystemcell一抗

Abcam  ab11326Mouse monoclonal [100/2] to alpha Adaptin100ul3996alpha Adaptinhystemcell一抗

Abcam  ab11327Mouse monoclonal [100/1] to AP1+2 beta1 00ul3996beta 1/2 Adaptinhystemcell一抗

Abcam  ab11328Mouse monoclonal [100/3] to gamma Adaptin100ul3996gamma Adaptinhystemcell一抗

Abcam  ab11329Mouse monoclonal [AP180-I] to AP180100ul3996AP18hystemcell一抗

Abcam  ab1133Rabbit polyclonal to GABARAP100ul3996GABARAPhystemcell一抗

Abcam  ab11330Mouse monoclonal [C6G9] to Carcino Embryonic Antigen CEA200ul3996CEAhystemcell一抗

Abcam  ab11331Mouse monoclonal [TD.1] to Clathrin heavy chain100ul3996Clathrin heavy chainhystemcell一抗

Abcam  ab11333Mouse monoclonal [1B10] to Fibroblast Surface Protein 200ul3996Fibroblast Surface Proteinhystemcell一抗

Abcam  ab11334Mouse monoclonal [LN-5] to Macrophage200ul3996Macrophagehystemcell一抗

Abcam  ab11335Mouse monoclonal [45M1] to Gastric Mucin 200ul3996Mucin Gastrichystemcell一抗

Abcam  ab11336Rabbit polyclonal to Parvin alpha50ul3996Parvin alphahystemcell一抗

Abcam  ab11337Rabbit polyclonal to l Afadin100ul3996l Afadinhystemcell一抗

Abcam  ab11338Rabbit polyclonal to l+S Afadin 100ul3996l/S Afadinhystemcell一抗

Abcam  ab11339Mouse monoclonal [FA-5] to N Cadherin100ul3996N Cadherinhystemcell一抗

Abcam  ab1134Rabbit polyclonal to GABA B Receptor 2100ul3996GABA B Receptor 2hystemcell一抗

Abcam  ab11340Mouse monoclonal [GC-4] to N Cadherin100ul3996N Cadherinhystemcell一抗

Abcam  ab11342Mouse monoclonal [CH-19] to pan Cadherin - Plasma Membrane Marker （FITC）100ul3996Cadherin panhystemcell一抗

Abcam  ab11343Rabbit polyclonal to CASK100ul3996CASKhystemcell一抗

Abcam  ab11346Rabbit polyclonal to alpha 1 Catenin100ul3885alpha 1 Cateninhystemcell一抗

Abcam  ab11347Rabbit polyclonal to alpha 2 Catenin100ul3996alpha 2 Cateninhystemcell一抗

Abcam  ab11348Mouse monoclonal [15B8] to beta Catenin （Cy3 ®）100ul3996beta Cateninhystemcell一抗

Abcam  ab11349Mouse monoclonal [BC-76] to beta Catenin （phospho S33）50ul3996beta Cateninhystemcell一抗

Abcam  ab1135Rabbit polyclonal to GABA B Receptor 1100ul3996GABA B Receptor 1hystemcell一抗

Abcam  ab11350Mouse monoclonal [BC-22] to beta Catenin （phospho S33+S37）50ul3996beta Cateninhystemcell一抗

Abcam  ab11351Rabbit polyclonal to delta 2 Catenin100ul3996delta 2 Cateninhystemcell一抗

Abcam  ab11352Rabbit polyclonal to delta 2 Catenin100ul3996delta 2 Cateninhystemcell一抗

Abcam  ab11354Mouse monoclonal [4C3] to HMGB150ug3996HMGB1hystemcell一抗

Abcam  ab11355Mouse monoclonal [ICRF44] to CD11b50tests3996CD11bhystemcell一抗

Abcam  ab11356Mouse monoclonal [ICRF44] to CD11b （FITC）50tests3996CD11bhystemcell一抗

Abcam  ab11358Mouse monoclonal [A3D8] to CD4450tests3996CD44hystemcell一抗

Abcam  ab11359Mouse monoclonal [8.4A6] to ICAM1100ul3996CD54,ICAM1hystemcell一抗

Abcam  ab1136Rabbit polyclonal to Artemin100ul3996Arteminhystemcell一抗

Abcam  ab11364Mouse monoclonal [FMC46] to CD62L100ul3996CD62Lhystemcell一抗

Abcam  ab11365Mouse monoclonal [FMC-46] to CD62L （FITC）50tests3996CD62Lhystemcell一抗

Abcam  ab11366Mouse monoclonal [CXN-32] to Connexin 32/GJB150ul3996Connexin 32,GJB1hystemcell一抗

Abcam  ab11367Rabbit polyclonal to Connexin 32/GJB1100ul3996Connexin 32,GJB1hystemcell一抗

Abcamab11327Mouse monoclonal [100/1] to AP1+2 beta1 00ul3996beta 1/2 Adaptin抗體一抗

Abcamab11328Mouse monoclonal [100/3] to gamma Adaptin100ul3996gamma Adaptin抗體一抗

Abcamab11329Mouse monoclonal [AP180-I] to AP180100ul3996AP18抗體一抗

Abcamab1133Rabbit polyclonal to GABARAP100ul3996GABARAP抗體一抗

更多詳情，請(qǐng)：     

重慶市華雅干細(xì)胞技術(shù)有限公司